By Naohiro Terasaka
In this thesis, functions of aminoacylation ribozymes named flexizymes are defined. Flexizymes have the subsequent specific features: (i) substrate RNA is famous via consecutive base pairs among the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) those base pairs should be substituted with different base pairs; and (iii) quite a few activated amino acids can be utilized as substrates together with either canonical and noncanonical amino acids. this versatile aminoacylation of RNAs by way of flexizymes was once used to label endogenous tRNAs to be got rid of, and in vitro choice utilizing the tRNA-depleted library enabled the invention of the radical interplay among the microRNA precursor and metabolites. Flexizymes also are used to arrange a variety of aminoacyl-tRNAs bearing mutations on the 3'-end to engineer the interpretation equipment and to strengthen the orthogonal translation equipment.
The first a part of the learn established that SELEX is suitable for locating the interplay among small RNA and ligands, and urged that extra RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new possibilities for in vitro man made biology concerning the engineering of the genetic codes and translation machineries. This learn additionally indicated the nice capability of aminoacylation by means of flexizymes to be utilized in quite a few fields of RNA learn, that is priceless for RNA researchers.
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Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA (Springer Theses) by Naohiro Terasaka